Genetic Engineering - Basics, Examples, Techniques in Human
Basics of genetic engineering Genetic engineering is the process of transferring specific genes from the chromosome of one organism and transplanting them into the chromosome of another organism in such a way that they become a reproductive part of the new organism. In Genetic Engineering, The process that produces the resulting recombinant DNA involves four steps: The desired DNA is cleaved from the donating chromosome by the action of restriction enzymes, which recognize and cut specific nucleotide segments, leaving a “sticky end” on both ends. The restriction enzymes also splice the receiving chromosome in a complementary location, again leaving “sticky ends” to receive the desired DNA. The desired DNA fragment is inserted into a vector, usually a plasmid, for transfer to the receiving chromosome. Plasmids are an ideal vector because they replicate easily inside host bacteria and readily accept and transfer new genes. Plasmids are circular DNA molecules found in the cytoplasm ...